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blasticidin selection  (Addgene inc)


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    Structured Review

    Addgene inc blasticidin selection
    Blasticidin Selection, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pclx+ubi+gfp/pCLX-UBI-GFP+(Plasmid+%2327245)/pm36602863-272-5-8
    Average 91 stars, based on 4 article reviews
    blasticidin selection - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Construct:

    Article Title: Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior
    Article Snippet: .. A first class of constructs contains a single expression unit, with one promoter governing the transcription of one single gene, as in pCLX-UBI-GFP (GFP, #27245, Addgene), pCLX-UBI-Tred (RFP, #27246, Addgene) and pCLX-M38TOP-dGFP (TOPdGFP). ..

    Article Title: Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior.
    Article Snippet: .. A first class of constructs contains a single expression unit, with one promoter governing the transcription of one single gene, as in pCLX-UBI-GFP (GFP, #27245, Addgene), pCLX-UBI-Tred (RFP, #27246, Addgene) and pCLX-M38TOP-dGFP (TOPdGFP). ..

    Expressing:

    Article Title: Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior
    Article Snippet: .. A first class of constructs contains a single expression unit, with one promoter governing the transcription of one single gene, as in pCLX-UBI-GFP (GFP, #27245, Addgene), pCLX-UBI-Tred (RFP, #27246, Addgene) and pCLX-M38TOP-dGFP (TOPdGFP). ..

    Article Title: Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior.
    Article Snippet: .. The first type contains a single expression unit, which is composed of one promoter driving one gene: pCLX-UBI-GFP (Addgene #27245), pCLX-UBI-TOM (tdTomato), pCLX-M38TOP-dGFP (dGFP driven by a synthetic TCF/LEF responsive promoter, derived from Addgene plasmid #17114). .. The second type of vector contains two transcription units, one with PGK promoter driving GFP and one with UBI promoter driving the GOI: pCWXPG-NT3; pCWXPG-hM3Dq.

    Article Title: Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior.
    Article Snippet: .. A first class of constructs contains a single expression unit, with one promoter governing the transcription of one single gene, as in pCLX-UBI-GFP (GFP, #27245, Addgene), pCLX-UBI-Tred (RFP, #27246, Addgene) and pCLX-M38TOP-dGFP (TOPdGFP). ..

    Selection:

    Article Title: Targeting HuR-Vav3 mRNA interaction prevents Pseudomonas aeruginosa adhesion to the cystic fibrosis airway epithelium
    Article Snippet: Briefly, the cells were seeded at 6 × 10 4 and infected with the control lentiviral vector, pCLX-Ubi-GFP, or the vector coding for TTP, pCWPGKBSD-TTP, at a multiplicity of infection (MOI) of 1 in the presence of Polybrene (Sigma-Aldrich) for 4 hours at 37°C in a humidified 5% CO 2 atmosphere. .. This step was followed by Blasticidin selection. pCLX-UBI-GFP (Addgene plasmid 27245; http://n2t.net/addgene:27245 ; RRID: Addgene_27245) and pCWPGKBSD-TTP were gifts from Patrick Salmon. .. HuR silencing in CFTR KD Calu-3 cells was obtained using the Tet-On inducible expression system.

    Article Title: Targeting HuR-Vav3 mRNA interaction prevents Pseudomonas aeruginosa adhesion to the cystic fibrosis airway epithelium.
    Article Snippet: Briefly, the cells were seeded at 6 × 104 and infected with the control lentiviral vector, pCLX-Ubi-GFP, or the vector coding for TTP, pCWPGKBSD-TTP, at a multiplicity of infection (MOI) of 1 in the presence of Polybrene (Sigma-Aldrich) for 4 hours at 37°C in a humidified 5% CO2 atmosphere. .. This step was followed by Blasticidin selection. pCLX-UBI-GFP (Addgene plasmid 27245; http://n2t.net/addgene:27245; RRID: Addgene_27245) and pCWPGKBSD-TTP were gifts from Patrick Salmon. .. HuR silencing in CFTR KD Calu-3 cells was obtained using the Tet-On inducible expression system.

    Plasmid Preparation:

    Article Title: Targeting HuR-Vav3 mRNA interaction prevents Pseudomonas aeruginosa adhesion to the cystic fibrosis airway epithelium
    Article Snippet: Briefly, the cells were seeded at 6 × 10 4 and infected with the control lentiviral vector, pCLX-Ubi-GFP, or the vector coding for TTP, pCWPGKBSD-TTP, at a multiplicity of infection (MOI) of 1 in the presence of Polybrene (Sigma-Aldrich) for 4 hours at 37°C in a humidified 5% CO 2 atmosphere. .. This step was followed by Blasticidin selection. pCLX-UBI-GFP (Addgene plasmid 27245; http://n2t.net/addgene:27245 ; RRID: Addgene_27245) and pCWPGKBSD-TTP were gifts from Patrick Salmon. .. HuR silencing in CFTR KD Calu-3 cells was obtained using the Tet-On inducible expression system.

    Article Title: Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior.
    Article Snippet: .. The first type contains a single expression unit, which is composed of one promoter driving one gene: pCLX-UBI-GFP (Addgene #27245), pCLX-UBI-TOM (tdTomato), pCLX-M38TOP-dGFP (dGFP driven by a synthetic TCF/LEF responsive promoter, derived from Addgene plasmid #17114). .. The second type of vector contains two transcription units, one with PGK promoter driving GFP and one with UBI promoter driving the GOI: pCWXPG-NT3; pCWXPG-hM3Dq.

    Article Title: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector
    Article Snippet: .. LTatC[M] was synthesized by GeneArt (Thermo Fisher Scientific), except that the HIV-1 Rev response element (RRE) was amplified and cloned from pEV731 (kindly provided by Eric Verdin) with primer pair 5′NNNNNNGTCGACCTCGAGATGGGTGCGAGAGCGTCAG3′ and 5′NNNNNNGTCGACGGTGGCATCGATACCGTCGAG3′, and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) was amplified from pCLX-UBI-GFP (kindly provided by Patrick Salmon; Addgene plasmid # 27245) with primer pair 5′NNNNNNGGATCCCGATAATCAACCTCTGGATTAC3′ and 5′NNNNNNACCGGTAATTCCCAGGCGGGGAGG3′. ..

    Article Title: Targeting HuR-Vav3 mRNA interaction prevents Pseudomonas aeruginosa adhesion to the cystic fibrosis airway epithelium.
    Article Snippet: Briefly, the cells were seeded at 6 × 104 and infected with the control lentiviral vector, pCLX-Ubi-GFP, or the vector coding for TTP, pCWPGKBSD-TTP, at a multiplicity of infection (MOI) of 1 in the presence of Polybrene (Sigma-Aldrich) for 4 hours at 37°C in a humidified 5% CO2 atmosphere. .. This step was followed by Blasticidin selection. pCLX-UBI-GFP (Addgene plasmid 27245; http://n2t.net/addgene:27245; RRID: Addgene_27245) and pCWPGKBSD-TTP were gifts from Patrick Salmon. .. HuR silencing in CFTR KD Calu-3 cells was obtained using the Tet-On inducible expression system.

    Derivative Assay:

    Article Title: Transient Deregulation of Canonical Wnt Signaling in Developing Pyramidal Neurons Leads to Dendritic Defects and Impaired Behavior.
    Article Snippet: .. The first type contains a single expression unit, which is composed of one promoter driving one gene: pCLX-UBI-GFP (Addgene #27245), pCLX-UBI-TOM (tdTomato), pCLX-M38TOP-dGFP (dGFP driven by a synthetic TCF/LEF responsive promoter, derived from Addgene plasmid #17114). .. The second type of vector contains two transcription units, one with PGK promoter driving GFP and one with UBI promoter driving the GOI: pCWXPG-NT3; pCWXPG-hM3Dq.

    Synthesized:

    Article Title: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector
    Article Snippet: .. LTatC[M] was synthesized by GeneArt (Thermo Fisher Scientific), except that the HIV-1 Rev response element (RRE) was amplified and cloned from pEV731 (kindly provided by Eric Verdin) with primer pair 5′NNNNNNGTCGACCTCGAGATGGGTGCGAGAGCGTCAG3′ and 5′NNNNNNGTCGACGGTGGCATCGATACCGTCGAG3′, and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) was amplified from pCLX-UBI-GFP (kindly provided by Patrick Salmon; Addgene plasmid # 27245) with primer pair 5′NNNNNNGGATCCCGATAATCAACCTCTGGATTAC3′ and 5′NNNNNNACCGGTAATTCCCAGGCGGGGAGG3′. ..

    Amplification:

    Article Title: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector
    Article Snippet: .. LTatC[M] was synthesized by GeneArt (Thermo Fisher Scientific), except that the HIV-1 Rev response element (RRE) was amplified and cloned from pEV731 (kindly provided by Eric Verdin) with primer pair 5′NNNNNNGTCGACCTCGAGATGGGTGCGAGAGCGTCAG3′ and 5′NNNNNNGTCGACGGTGGCATCGATACCGTCGAG3′, and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) was amplified from pCLX-UBI-GFP (kindly provided by Patrick Salmon; Addgene plasmid # 27245) with primer pair 5′NNNNNNGGATCCCGATAATCAACCTCTGGATTAC3′ and 5′NNNNNNACCGGTAATTCCCAGGCGGGGAGG3′. ..

    Clone Assay:

    Article Title: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector
    Article Snippet: .. LTatC[M] was synthesized by GeneArt (Thermo Fisher Scientific), except that the HIV-1 Rev response element (RRE) was amplified and cloned from pEV731 (kindly provided by Eric Verdin) with primer pair 5′NNNNNNGTCGACCTCGAGATGGGTGCGAGAGCGTCAG3′ and 5′NNNNNNGTCGACGGTGGCATCGATACCGTCGAG3′, and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) was amplified from pCLX-UBI-GFP (kindly provided by Patrick Salmon; Addgene plasmid # 27245) with primer pair 5′NNNNNNGGATCCCGATAATCAACCTCTGGATTAC3′ and 5′NNNNNNACCGGTAATTCCCAGGCGGGGAGG3′. ..

    Virus:

    Article Title: Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector
    Article Snippet: .. LTatC[M] was synthesized by GeneArt (Thermo Fisher Scientific), except that the HIV-1 Rev response element (RRE) was amplified and cloned from pEV731 (kindly provided by Eric Verdin) with primer pair 5′NNNNNNGTCGACCTCGAGATGGGTGCGAGAGCGTCAG3′ and 5′NNNNNNGTCGACGGTGGCATCGATACCGTCGAG3′, and the Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) was amplified from pCLX-UBI-GFP (kindly provided by Patrick Salmon; Addgene plasmid # 27245) with primer pair 5′NNNNNNGGATCCCGATAATCAACCTCTGGATTAC3′ and 5′NNNNNNACCGGTAATTCCCAGGCGGGGAGG3′. ..



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